Improving the immunogenicity of a trivalent Neisseria meningitidis native outer membrane vesicle vaccine by genetic modification
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چکیده
منابع مشابه
Improved Production Process for Native Outer Membrane Vesicle Vaccine against Neisseria meningitidis
An improved detergent-free process has been developed to produce vaccine based on native outer membrane vesicles (NOMV) against Neisseria meningitidis serogroup B. Performance was evaluated with the NonaMen vaccine concept, which provides broad coverage based on nine distinct PorA antigens. Scalable aseptic equipment was implemented, replacing undesirable steps like ultracentrifugation, inactiv...
متن کاملbiological and immunological evaluation of neisseria meningitidis serogroup a outer membrane vesicle as vaccine candidates
conclusions the results showed that the extracted omvs were conformationally stable, and there were no pyrogenic determinants in omv. also the results showed that the omv elicited high level of specific antibodies against n. meningitidis serogroup a. these results indicate that the omv obtained here, can be used as a meningococcal vaccine after further investigation. materials and methods omvs ...
متن کاملImpact of Reducing Complement Inhibitor Binding on the Immunogenicity of Native Neisseria meningitidis Outer Membrane Vesicles
Neisseria meningitidis recruits host human complement inhibitors to its surface to down-regulate complement activation and enhance survival in blood. We have investigated whether such complement inhibitor binding occurs after vaccination with native outer membrane vesicles (nOMVs), and limits immunogenicity of such vaccines. To this end, nOMVs reactogenic lipopolysaccharide was detoxified by de...
متن کاملDevelopment of a Vaccine for Neisseria Meningitidis Group B Based on Native Outer Membrane Vesicles
A novel approach for development of a meningococcal group B vaccine has been developed. This approach , which uses vesicles or blebs of the meningococcal outer membrane prepared without exposure to detergent or denaturing solvents, allows the outer membrane antigens to be presented to the immune system in their natural conformation and membrane environment. Two strategies for using these native...
متن کاملCysteine Depletion Causes Oxidative Stress and Triggers Outer Membrane Vesicle Release by Neisseria meningitidis; Implications for Vaccine Development
Outer membrane vesicles (OMV) contain immunogenic proteins and contribute to in vivo survival and virulence of bacterial pathogens. The first OMV vaccines successfully stopped Neisseria meningitidis serogroup B outbreaks but required detergent-extraction for endotoxin removal. Current vaccines use attenuated endotoxin, to preserve immunological properties and allow a detergent-free process. The...
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ژورنال
عنوان ژورنال: Vaccine
سال: 2016
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2016.05.049